While quantification of viruses that cause important infections in transplant recipients has been the standard of care for years, important challenges related to standardization remain.

| Privacy Policy and Terms of Use. document.write(addy_text81834); The diagnosis of tuberculosis (TB) in HIV-infected patients is challenging. Our aim was to develop a simple, low-cost method of preparing DNA for sequencing direct from M.... QuantiFERON-TB Gold Plus (QFT-Plus) is the most widely used interferon gamma release assay (IGRA) for the diagnosis of latent tuberculosis infection (LTBI).

The patients were subsequently classified into two groups according to the presence of complications. However, culture may lack sensitivity, and some enteropathogens, such as pathovars of Escherichia coli, may escape routine diagnosis. BioMérieux has recently recalled some of their antibiotic susceptibility testing (AST) cards for gram-positive bacteria using the VITEK 2 system. More About ASM Journals Take advantage of improved member benefits that will help you advance. 04-21-2019, Laboratory Diagnosis of Parasites from the Gastrointestinal Tract:This Practical Guidance for Clinical Microbiology document on the laboratory diagnosis of parasites... Postdoc through ASM’s Committee on Postgraduate Education Programs (CPEP) (optional but desirable), or informal training for 3 years in a clinical microbiology lab, or M.D. However, how to optimally integrate Xpert and LAM tests into clinical practice algorithms remain unclear. In this study, we report the analytical and clinical performance characteristics of a new, high-throughput, fully automated nucleic acid amplification test system for the detection of SARS-CoV-2. Interventions to optimize blood culture (BCx) practices in adult inpatients are limited.

Targeted next-generation sequencing (tNGS) has emerged as a comprehensive alternative to existing methods for drug susceptibility testing (DST) of Mycobacterium tuberculosis from patient sputum samples for clinical diagnosis of drug-resistant tuberculosis (DR-TB). A Case of Fascioliasis with Pulmonary Involvement in a Peace Corps Volunteer, Closing the Brief Case: What a Fluke!

Despite decreasing costs, next-generation sequencing (NGS) is still prohibitively costly for routine use in generalized HIV epidemics in low- and middle-income countries.

1752 N St. NW Diagnostic Accuracy of Interferon Gamma-Induced Protein 10 mRNA Release Assay for Tuberculosis, An Optimal Diagnostic Strategy for Tuberculosis in Hospitalized HIV-Infected Patients Using GeneXpert MTB/RIF and Alere Determine TB LAM Ag, Evaluation of MALDI Biotyper Interpretation Criteria for Accurate Identification of Nontuberculous Mycobacteria, A Comprehensive Genomics Solution for HIV Surveillance and Clinical Monitoring in Low-Income Settings, Evaluation of Six Commercial Mid- to High-Volume Antibody and Six Point-of-Care Lateral Flow Assays for Detection of SARS-CoV-2 Antibodies, A Recurrent Mutation at Position 26340 of SARS-CoV-2 Is Associated with Failure of the E Gene Quantitative Reverse Transcription-PCR Utilized in a Commercial Dual-Target Diagnostic Assay, Saliva Sampling and Its Direct Lysis, an Excellent Option To Increase the Number of SARS-CoV-2 Diagnostic Tests in Settings with Supply Shortages, Performance Characteristics of a High-Throughput Automated Transcription-Mediated Amplification Test for SARS-CoV-2 Detection, Development and Validation of the Elecsys Anti-SARS-CoV-2 Immunoassay as a Highly Specific Tool for Determining Past Exposure to SARS-CoV-2, Rapid Salivary IgG Antibody Screening for Hepatitis A, Comparison of Molecular Subtyping and Antimicrobial Resistance Detection Methods Used in a Large Multistate Outbreak of Extensively Drug-Resistant, Comparison of Extraction Methods and Thermocyclers for SARS-CoV-2 Molecular Detection Using Clinical Specimens, Erratum for Hernández et al., “Invasive Pneumococcal Disease and Influenza Activity in a Pediatric Population: Impact of PCV13 Vaccination in Epidemic and Nonepidemic Influenza Periods”, Working Group of Projects PI11/02081 and PI11/2345, Laboratory Diagnosis of COVID-19: Current Issues and Challenges, Performance Characteristics of the Abbott Architect SARS-CoV-2 IgG Assay and Seroprevalence in Boise, Idaho, Performance of Abbott ID Now COVID-19 Rapid Nucleic Acid Amplification Test Using Nasopharyngeal Swabs Transported in Viral Transport Media and Dry Nasal Swabs in a New York City Academic Institution, Saliva as a Noninvasive Specimen for Detection of SARS-CoV-2, Submission, Review, & Publication Processes, http://cge.cbs.dtu.dk/services/VirulenceFinder/, COVID-19 Research and News from ASM Journals. To answer questions regarding use of Vitek 2 AST cards, the ASM Clinical Laboratory Practice Subcommittee has provided guidance to aid clinical labs with this issue. 08-30-2018, ASM has partnered with CDC to develop a recently published guideline on Clostridium difficile. Recent trends show a decrease in anti-HAV antibodies in the general... Mycoplasma pneumoniae is a major cause of community-acquired pneumonia. Hepatitis A virus (HAV) is a common infection that is transmitted through the fecal-oral route, shed in the stool of infected individuals, and spread either by direct contact or by ingesting contaminated food or water. However, the complexity of sequencing platforms has limited their uptake in low-resource settings. Although highly specific, IGRAs have a relatively high false-negative rate in active TB patients.

A lack... Extraintestinal pathogenic Escherichia coli (ExPEC) is the leading cause in humans of urinary tract infection and bacteremia. Journal of Clinical Microbiology® (JCM) publishes the most current research related to the laboratory diagnosis of human and animal infections and the role of the laboratory in both the management of infectious diseases and the elucidation of the epidemiology of infections.

The COVID-19 pandemic caused by the new SARS-CoV-2 coronavirus has imposed severe challenges on laboratories in their effort to achieve sufficient diagnostic testing capability for identifying infected individuals. With over 90 years of experience, ASM journals continue to be an authoritative source for the latest in microbiology research. Traditional molecular subtyping methods, such as pulsed-field gel electrophoresis (PFGE) and 7-gene multilocus sequence typing (MLST), provided limited resolution to adequately identify C. jejuni outbreaks and... NEW:COVID-19 Research and News from ASM Journals.

The issues are wide ranging, and until they are adequately addressed, the full impact of viral load testing regarding clinical management decisions will not be realized.

We performed a head-to-head assessment of enzyme immunoassays (EIAs) and point-of-care lateral flow assays (POCTs) to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies. We evaluated sensitivity, specificity, cross-reactivity, and agreement with a vesicular stomatitis virus-based pseudoneutralization assay for the Elecsys Anti-SARS-CoV-2 immunoassay. We investigated the impact of rapid AST on clinical and antimicrobial stewardship outcomes in real-world practice. Identification of mycobacteria by matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) requires not only a good protein extraction protocol but also an adequate cutoff score in order to provide reliable results. //-->

Demonstrate expertise in medical and public health microbiology with ABMM certification; application deadline April 1. 02-27-2019. The aim of this study was to compare QFT-Plus results by enzyme-linked immunosorbent assay (ELISA) on the SkyLab system with those obtained with chemiluminescence immunoassay (CLIA) on the Liaison XL analyzer. American Society for Microbiology ("ASM") is committed to maintaining your Improved rapid diagnosis and antimicrobial resistance determination, such as by whole-genome sequencing, are required. A Case of Fascioliasis with Pulmonary Involvement in a Peace Corps Volunteer, Persistent Challenges of Interassay Variability in Transplant Viral Load Testing, Advances in Molecular Diagnosis of Tuberculosis, Detection and Characterization of Diphtheria Toxin Gene-Bearing, Imipenem-Relebactam Susceptibility Testing of Gram-Negative Bacilli by Agar Dilution, Disk Diffusion, and Gradient Strip Methods Compared with Broth Microdilution, Improved Early Detection of Focal Brucellosis Complications with Anti-, Performance Evaluation of the Novodiag Bacterial GE+ Multiplex PCR Assay, A Diagnostic Stewardship Intervention To Improve Blood Culture Use among Adult Nonneutropenic Inpatients: the DISTRIBUTE Study, Performance of Four Fosfomycin Susceptibility Testing Methods against an International Collection of Clinical, Application of Targeted Next-Generation Sequencing Assay on a Portable Sequencing Platform for Culture-Free Detection of Drug-Resistant Tuberculosis from Clinical Samples, DNA Thermo-Protection Facilitates Whole-Genome Sequencing of Mycobacteria Direct from Clinical Samples. Watch Parasites - Editors in Conversation Hosted by Journal of Clinical Microbiology Editor in Chief, Dr. Alexander McAdam.

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